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A student researcher performed a chromatographic separation of caffeine and aspartame. The retent...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine,...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine,...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc,was found to be 207.6 s with a baseline peak width, wc, of 16.2 s. The retention time for aspartame, ta, was 253.7 s with a baseline peak width, wa, of 18.7 s. The retention time for the unretained solvent methanol was 40.7 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.3 cmlong...
12. (16 pts) A researcher performed a chromatographic separation of caffeine and aspartame using methanol as...
12. (16 pts) A researcher performed a chromatographic separation of caffeine and aspartame using methanol as the mobile phase. The retention time for methanol was 20.05 The retention time for caffeine (t.) was found to be 215.2 s with a peak width (w.) of 11.5 s and the retention time for aspartame (t) was 276.8 s with a peak width (w.) of 21.8 s. A) Calculate the resolution (R) for this separation B) Calculate the retention factor (k) for aspartame.
1. Listed below are retention times and peak widths for an HPLC chromatographic separation on a...
1. Listed below are retention times and peak widths for an HPLC chromatographic separation on a 10 cm C18 column a) Calculate the resolution between benzene and benzo[e]pyrene. Compound Unretained Benzene Benzo[e]pyrene Pyrene Coronene Time (min) Baseline Width (min) 0.9 10.9 13.3 16.7 26.1 0.40 0.40 0.45 0.50 b) Is this a good resolution, yes, no, why? c) Calculate the theoretical number of plates for benzo[elpyrene.
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
need help with number 2 HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extr...
need help with number 2
HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extracted? For the same solute and, what fraction will be extracted if 5 extractions wi 80 mL chloroform each are used (instead of one 400 ml extraction)? M solution containing solute A in water is extracted one time with...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
12. (16 pts) A researcher performed a chromatographic separation of caffeine and aspartame using methanol as the mobile phase. The retention time for methanol was 20.05 The retention time for caffeine (t.) was found to be 215.2 s with a peak width (w.) of 11.5 s and the retention time for aspartame (t) was 276.8 s with a peak width (w.) of 21.8 s. A) Calculate the resolution (R) for this separation B) Calculate the retention factor (k) for aspartame.
1. Listed below are retention times and peak widths for an HPLC chromatographic separation on a 10 cm C18 column a) Calculate the resolution between benzene and benzo[e]pyrene. Compound Unretained Benzene Benzo[e]pyrene Pyrene Coronene Time (min) Baseline Width (min) 0.9 10.9 13.3 16.7 26.1 0.40 0.40 0.45 0.50 b) Is this a good resolution, yes, no, why? c) Calculate the theoretical number of plates for benzo[elpyrene.
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
need help with number 2
HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extracted? For the same solute and, what fraction will be extracted if 5 extractions wi 80 mL chloroform each are used (instead of one 400 ml extraction)? M solution containing solute A in water is extracted one time with...
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