Question

1. Listed below are retention times and peak widths for an HPLC chromatographic separation on a 10 cm C18 column a) Calculate the resolution between benzene and benzo[e]pyrene. Compound Unretained Benzene Benzo[e]pyrene Pyrene Coronene Time (min) Baseline Width (min) 0.9 10.9 13.3 16.7 26.1 0.40 0.40 0.45 0.50 b) Is this a good resolution, yes, no, why? c) Calculate the theoretical number of plates for benzo[elpyrene.
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Answer #1

Perk ead peak Ri (min) 0 4 width (min) 3-3-1o- 6.0 2 benzo ( e)emen 2 13. 3 16 N = 689

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