Question

DNA: Synthetic Yields 4 of 15 > Part A If a DNA trimer is synthesized using the solid-phase phosphoramidite method starting from an initial immobilized nucleoside analog with 90% yield in each subsequent coupling step, what is the overall percent yield? (provide only the numerical answer, do not include the percent sign) AEP O P ? Submit Request Answer Part B If a DNA trimer is synthesized using the solid-phase phosphoramidite method starting from an Initial Immobilized nucleoside analog with 64% overall yleld, what was the percent yield in each of the coupling steps? (provide only the numerical answer, do not include the percent sign) V AXPO W? Submit Request Answer

Answer 1

yan TOO T for synthesis of DNA we need two steps Buta. DNA TO DNA + BAO DNA, Dame DNA - ONA, MONng E het us slaut we'lli a or be for step I s 90% of x = 0.92 Fo Baht The yield for step wohl be 90 of 0.9% = 0.8] ? Querau yield - 0.814. (formea) 100 = 31% ! Part B I Let us start with be y gm . | % f each slip be zool gor step o yield is so my mom for step pield is 39 62 - Todo - Coriginal De 4 am , * annall gield, 2 Qurall nie 1000 1100 - 2 1000 2100 = 2 100 Gineu, Too = 64 % + 22 = 64x100 - z=26400 = 180