Question

The graph below shows the results of a hypothetical XTT assay comparing the XTT absorbance from two cell lines as a function of cell number. Absorbance 450 nm NIH3T3 cells mmmmm(H) 1 . 10.00 20.05 0.05 1006 121406 0.05 0.05 Cell number a. Choose a potential explanation as to why the CHO curve sits below the NIH3T3? CHO cells have less proteins than NIH-3T3 cells CHO cells are smaller than NIH-3T3 cells CHO cells have less reducing enzymes than NIH-3T3 cells CHO cells have more mitochondria than NIH-3T3 cells NiH-3T3 cells have less reducing enzymes than CHO cells Submit Answer Tries 0/99 b. A set of unknown" NIH3T3 and CHO cells were plated at a concentration of 2 x 10" cells per well and grown for two days prior to the XTT assay. Just as you did in week 7, these "unknown" wells were included in the above XTT assay. Both "unknown" wells gave an absorbance of 0.5. Using the standard curve in the above graph, what would be the estimate number of cells in the unknown wells after two days of growth? (Enter your results in scientific notation) NIH-3T3: cells Submit Answer Tries 0/99 CHO: cells

Answer 1

a.

The most possible reason for the obtained results in the graph is likely due to-

i) CHO cells have less reducing enzyme than NIH3T3 cell

b.

The cell no can be calculated by using the above graph

The estimated cell number will be 6 * 105