We can expect a sample of soil taken from a garden to be positive for nitrate reductase (NR). The detection of this enzyme is reported (Abdelmagid and Tabatabai, 1986). It is also reported that the inactivation of nitrate reductase can be achieved at 40 degree celsius temperature and complete destruction by steam sterilization.
Nitrate reductase is an enzyme that catalyses the reduction of nitrate to nitrite. Nitrate is a naturally occuring form of nitrogen in soil, which is created by nitrification from ammonia. NR activity can be estimated by measuring the amount of nitrite being produced from nitrate .
1. Would you expect a sample of soil taken from a garden to be positive for...
You have successfully isolated pure cultures of a bacterium from soil. What result might you expect if you incolauted it into nitrate broth and why? How would hou determine this result? Please be specific.
A sample was taken with 15 observations of soil tests from North Carolina rural yards. We would like to determine whether there is reason to believe the nitrate levels differ from the national average. Our sample average shows 8.19 parts per million (ppm) nitrate concentration with a sample standard deviation of 3.95 ppm. Suppose the national average for rural yard is 6.25 ppm. Conduct the hypothesis test using a 90% confidence level and answer the following questions. For all numbers,...
The mass of DNA collected from the compost soil was 1.523 x10-6g and from the garden soil was 3.767 x10-6g. Original mass of compost soil = 0.222g Original mass of garden soil = 0.27g Assuming: 1) 100% recovery of the cellular DNA from your bacteria; and 2) the mass of DNA in a single bacterium weighs 5.2·10-15 g (true for E. coli at least) ... how many bacteria were present in your soil sample? How many per g of soil?...
A soil profile is shown below. The soil sample is taken from depth of 11m, a) Is it OC or NC soil? b) If it is loaded with A6= 28 t/m2 calculate the settlement of clay layer. For sand Ydry = 1.75 t/m and Ysat = 2.0 t/m3 For clay Xsat = 1.9 t/m3 6'o+A6 SC 6'o+A6 CsH Сен -log 1+ Sc log 087645) 610 1+eo 610 Sc CH 1+0 -log 6) + CCH 1+ -log (9729) 610 Sand 5m...
1. A soil sample is taken from a small test hole in a soil borrow pit. The volume of the test hole is 5 ft. The wet mass of the soil is 18 kg and the dry mass is 21 kg. The specific gravity of the soil solids is 2.65. ( m 35. 3147ft)(1 kge 2.205 lb) ( 1 lb= 0.2248 N) Determine: a. Wet and dry density (Mg/m) b. Wet and dry unit weight (pcf) C. Water content (%)...
Would you expect the dipeptide valylalanine to give a positive reaction with nitric acid solution? Why or why not? 3, Would you expect the dipeptide valylalani Why or why not? alanine to give a positive reaction with nitric acid solution? 4. In the chromatogram below, calculate an Re for each component of the mixture. Which componert is probably the more polar compound? Solvent front Origin From a book or Internet source, find the pl of the amino acid lysine. What...
Why would a soil sample tested with CaCl2 have a more acidic pH than a soil sample tested woth DI water?
You want to start a new garden but your soil looks like beach sand and your soil test is low in most nutrients. From a soil chemistry point of view, what are the advantages of adding nutrients through compost rather than an inorganic fertilizer? Discuss short-term and long-term benefits.
If all soil bacteria on Earth were to suddenly disappear, which nutrient cycle would you expect to be the most strongly affected
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...