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1. Would you expect a sample of soil taken from a garden to be positive for nitrate reductase? Why or why not?
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We can expect a sample of soil taken from a garden to be positive for nitrate reductase (NR). The detection of this enzyme is reported (Abdelmagid and Tabatabai, 1986). It is also reported that the inactivation of nitrate reductase can be achieved at 40 degree celsius temperature and complete destruction by steam sterilization.

Nitrate reductase is an enzyme that catalyses the reduction of nitrate to nitrite. Nitrate is a naturally occuring form of nitrogen in soil, which is created by nitrification from ammonia. NR activity can be estimated by measuring the amount of nitrite being produced from nitrate .

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