Question

9:13 1 Search HD Lab 10 Chromatography S20 Onl... 1150 HD 10 Page 1 of 3 Biol 1150 HD 10 Chromatography and Plant pigments 1. When you set up a paper chromatography sample, what do you think would happen if the level of solvent in the tube was higher than the origin line? 2. Assume that 2 of the leaf pigments on your chromatogram did not completely separate, how could you modify the procedure to completely separate those 2 pigments? 3. Calculate the Rf value for the pigments on this chromatogram. P rvale 4. Analyze the provided paper chromatography data to calculate Rf values. Table 3 Dashboard Calendar To Do Notifications inbox

Answer 1

Ans.1- If the level of solvent in the tube was higher than the origin line then the solutes pigments continue to move up and all different solute pigments will merge together at the top of the stationary phase of paper chromatography. And, this will cause error in result of paper chromatography. In paper chromatography, different solute samples are separated between mobile phase and stationary phase. Mobile phase is solvent used and stationary phase is that on which pigments samples are loaded.

Ans.2- If two of the leaf pigments on our chromatogram did not completely separate then to increase solute retention factor of that 2 pigments use a mobile phase that is weaker solvent. Because if mobile phase has a lower solvent strength, then solute spend more in the stationary phase and separate properly.

Ans.3- Rf value is the retention factor. Rf value of individual solute can be find out by dividing distance traveled by solute to distance traveled by solvent. That means Rf value is the ratio of distance traveled by solute to distance traveled by solvent.

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