and how to heal our results? no copy-paste, please.
this is our result and there are some blue colors at the bottom and no white color .
There is blue colonies observed in LB+AMPICILLIN plate for transformed competent cells when compared to the control competent cells that means transformation of plasmid with ampicilin resistance gene occured. Hence there has been growth observed .
There are no white colonies observed means ligation of insert with plasmid did not occur and it is self ligated before transformation. Hence no recombination occured and insert is not transformed but only the plasmid
and how to heal our results? no copy-paste, please. this is our result and there are...
explain the results for your own group and compare to other groups -especially if a group has a different result than yours , discuss possible errors?did the transformation occur? how do you come to this conclusion ? did you observe the alpha complementation or not? what do the control plates prove to you?did you see only blue colonies or were there any white ones as well and why? and how to heal our results? no copy-paste, please. this is our...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was added into the tube which contains competent cells and the tube was tapped gently to mix DNA and the competent bacteria. After that it was placed on ice for 30 minutes. Then, the tube with the competent bacteria and plasmid were transferred to heating block at 42 °C and the tube was leaved in there exactly 90 seconds. 0.25 ml of LB broth was...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was added into the tube which contains competent cells and the tube was tapped gently to mix DNA and the competent bacteria. After that it was placed on ice for 30 minutes. Then, the tube with the competent bacteria and plasmid were transferred to heating block at 42 °C and the tube was leaved in there exactly 90 seconds. 0.25 ml of LB broth was...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Need help filling in the chart and answering the questions that go along with it. I have added the procedure and the instructions as well as the "results" that are supposed to be used to fill in the chart. Thank you! We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...