1. Much of an individual’s DNA is identical to others, and indeed shows strong homology to many other species. What types of sequences would you predict would be highly conserved?
2.In the lab session you will isolate DNA from your check cells, using a sterile isotonic saline wash.What is the most likely contaminating DNA when you sample by this method?
3. Will this matter for what we are trying to achieve, namely amplify a hypervariable region on chromosome 1? Explain your response.
4. What contamination will confound your results?
1. Highly conserved sequences may include those which can encode functions of cell, such as some genes for various digestive enzymes, ribosomes and those involved in the creation of some physical characteristics, such as our hands, and organs that serve specific functions for body.
2.In the lab session the isolation of DNA from the check cells, using a sterile isotonic saline wash.The most likely contaminating DNA by this method is the Bacterial DNA which is collected indiscriminantly.
3. Hypervariable regions are sections of DNA that vary greatly between individuals they can be used to distinguish people based on their DNA.Collection of DNA from bacteria will most likely have no impact on the result of the experiment. This is because the primer being made is very specific to the region of DNA we wish to amplify. As such, as long as this sequence is not present in the bacterial genome, the presence of this extra DNA is not affected on the result.
4.Other human DNA will confound the results(the person doing the experiment ,etc).This is why gloves must always be worn to prevent samples of another person's DNA affecting the sample.
1. Much of an individual’s DNA is identical to others, and indeed shows strong homology to...