Question

Total question is worth 10 marks A1. Answer ALL parts: In insulin-responsive cells the insulin receptor substrate 1 (IRS-1) protein is believed to be a major component of signal transduction. Several experiments were performed to investigate the relationship between IRS-1 and three adaptor proteins (Grb2, Shc, and p85) with respect to their role in insulin signaling. The descriptions of experiments are provided in the legends to the figures. Use Figures 1, 2 and 3 to provide the most appropriate answer. Fra 1. Prot a ract were prepared from untre treated (+) calle w a ar b ample -2-1 emples and bodies The pred proteine were tractioned by 808 polymorylamide electrophore and the Western blok mely permed cry -RS-1 p wd . phoephotyroene we were ind 4. orand-Gube names and antibodies MU moleouter masses of marker wine in Mall

Answer 1

a) 1 The Statemen Is supported by the data given

b)1The Statemen is supported by data given

c)1 The Statemen is supported by data given

d)2 The statemen contradicted by the data given

e)1The Statemen is supported by data given

f)3 The statemen neither supported nor contradicted by the data given

g)2 The statemen contradicted by the data given

h)1The Statemen is supported by data given

i) 2 The statemen contradicted by the data given

j) 3The statemen neither supported nor contradicted by the data given