Question

how can we tell if a DNA molecule is homo or homeologous without siquencing it ? (Short answer)

Answer 1

We can go for DNA hybridization.DNA hybridization is the process of joining two single strands of DNA thus forming double stranded DNA. Joining of the two strands is facilitated by the formation of hydrogen bonds between the nucleotide bases. This hybridization procedure can be used to assess the genetic similarity between two populations of DNA or to detect a specific sequence of DNA in a population of DNA molecules.

uses

1)To determine relatedness between strains

2)Delineation of bacterial species

diagrammatic representation of DNA hybridization

Organism y own organism X DNA ISTITUTI IIIII ③ Applying heat to separate strand T TTTTTT !! ! ] VILL T TTT combining to LILLLL od DNA two strands ② combining Cool to allow denaturation od ds DNA Determine degree . of hybridisation a) HIITTI6) ILI 9 TIITT complete hybridisation partial No hybridisation hybridisation x andy are identical unrelated X and Y are related