Question

the autoclave collection cart. Dispose of gloves in the appropriate waste receptacle. Questions 1. What is the purpose of adding mineral oil to an O/F Media tube and why do we put mineral oil in nly one? 2. What additional information must be known about Phenol Red Broth? . Explain how conducting the MRVP test too soon in a cultures growth cycle can lead to false negatives? 7. Generally, the color change we see in these Sests is a result of what change in the media? Explain References Jottschalk, Gerhard. 1986. Bacterial Metabolism. Second Edition. Springer-Verlag, New York Inc. Hew York, New York. Difco Manual. 1984. Difco Laboratories ncorporated, Detroit, Michigan 48232

Answer 1

1. A layer of mineral oil is added to the top of the deep in one of the tubes to create anaerobic conditions.

2. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram-negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate.

3. MRVP test is designed to detect organisms capable of performing a mixed acid fermentation, which overcomes the phosphate buffer in the medium and lowers the pH. Conducting the MRVP test too soon results in inhibited bacterial growth that causes false-negative results.

4.