Question

Lab Notebook Measured Distance (mm) Actual Base Pairs (bp) 23,130 16 9416 6557 4361 2322 18 22 29 32 46 2207 564 Practice Problem 1 Distance Migrated (mm) Interpolated Fragment Size (bp) 4796 Fragment 1 Fragment 2 1405 Fragment 3 1268 Practice Problem 2 Distance Migrated (mm) Interpolated Fragment Size (bp) 6201 1268 Fragment 1 Fragment 2 1. What is the relationship of migration distance to fragment size? RESPONSE:

Answer 1

Migration distance is inversely proportional to the fragment size. Smaller fragments migrate higher distance from sample well in the gel than the larger fragments (higher base pairs). As seen from the graph below

30000 25000 20000 y = 47790e-0.099x R= 0.9688 Actual base pair (bp) 15000 10000 5000 0 10 40 50 20 30 Measured distance (mm)

Actual base pair (bp) y=47790e 0.09% R = 0.9688 1 10 40 50 20 30 Measured distance (mm)

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