Question

Flc 2: Gram precipit Gram Selective for: Gram- enterics Bile salts and crystal viole gram+ Differentiates between lactose and non lactose Neutral red dye is an indicator is a p indicator -organisms that ferment lacto lower the pH and colonies turn red kish, has bile ose negative) Ye You suspect a bacterial contamination on your culture plate. A. What would you do next and b. why? the Bonus: HD B1. How many bacteria will be on plate A, 05 How many bacteria do you expect on plate B, and C. What is the dilution factor of plate B? 9.9mL 9.0mL 1000L 1000L 1.0mL 1.0mL YFB O/N 102 Pipette 104 2.0x10 10-5 10-6 100ul unto all plates C. What is the final dilution here. or flask) that will hold at least twice the arents according to the recipe of your choice. with aluminum foil, or plastic wrap if you use a microwave. emble the ho gra bala tools plates glass cor e of your n he methods do 3.
please answer both questions

Answer 1

CFU/ mer colonies volume pealed x dilution 100 u=0./me for A, 2X10 9 = colonies 01x107 colonies = 2x109xo1x10-5 = dooo for B, LX109= colonies 1 01x10-6 opules = axbºx (0-6 xo:| = doo dilun factor = n | o 1087 final dilums 0.1x10-6 (volume plated X tube dilum)

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