Why are we treating our purified PCR products with a DNA polymerase before adding them to the ligation reaction?
and
What is the purpose of ATP in the ligation reaction?
Group of answer choices
-It provides energy to the ligase to catalyze a peptide bond
-It provides energy to the ligase to catalyze a phosphodiester bond
-It provides energy to the ligase to cut the DNA
-It provides energy to the ligase to unwind the DNA
DNA polymerase added to the PCR product before ligation so that there is found synthesis of all complementary base pair in the newly synthesised segment of DNA.
The ATP is added because it provides the energy to the ligase to catalyse a phosphodiester bond.
Why are we treating our purified PCR products with a DNA polymerase before adding them to...