Question

15. Safety precautions used in the laboratory include all of the following except: A. lab personnel must keep hair length short B. wear protective gloves C. wear laboratory coat D. open toed shoes are not be worn in the lab 16. Human Chorionic Gonadotropin is comprised of 2 subunits. What subunit is the specific indicator of a positive test. a. alpha b. beta 17. A constant amount of antigen is mixed with a serially diluted patient's serum. Eventually there is a point where agglutination disappears. This is known as: a. zone of equivalence b. prozone C. post zone d. negative result 18. In traditional testing for syphilis, a positive RPR test confirms a diagnosis of syphilis. (T or F)

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Answer 1

15. Answer is a

16. Answer is b

Beta subunit is specific for hCG and alpha subunit is identical for LH, FSH and TSH

17. Answer is c

Here the amount of antigen is constant and the sample is serially diluted. As a result of serial dilution the concentration of antibody falls down at some point. The high concentration of antigen results in desappeance of agglutination.

Optimum amount of antigen and antibody is present in zone of equivalence.  

Excess amount of antibody and no agglutination is prozone.

18. Answer is False

Traditional positive RPR test cannot confirm syphilis as this test gives positive result if a person is having malaria, HIV etc. Further tests like fluorescent treponemal antibody absorption test are needed for the confirmation of syphilis.