Answer:
Refer to the images below for the handwritten answer:
Note: This equation is formed by using the Rapid Equilibrium Assumption.
It can be done through another Assumption known as the Quasi-Steady State Assumption to obtain the same result.
(10 points) Michaelis-Menten kinetics. Consider ving enzymatic reaction, E + A H EA → E+ P...
4. Basic concepts of Michaelis-Menten kinetics. The Michaelis-Menten equation is expression of the relationship between the initial velocity, Vo, of an enzymatic reaction and substrate concentration, [S]. There are three conditions that are useful for simplifying the Michaelis-Menten equation: [S] <<Km; [S] = Km; [S] >> Km. Match each condition with the statement(s) that describe it. TV, Vmox[S] Vo =Vmax m . V Vo - Vmax [S] Km +[S] V. (um/min) max [S] (mm) (a) Doubling [S] will almost double...
For an enzyme that displays Michaelis-Menten kinetics, what is the reaction velocity v (as a percentage of Vmax) ,observed at each of the following substrate concentrations. (Ex, v = xVmax, where x = an integer, fraction, or decimal number (two decimal places)) a) [S] = 0.1 Km _________ b) [S] = 2 Km _________ c) [S] = 10 Km _________
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
please answer a,b and c. 4. Consider when enzyme E binds to Substrate Sassuming Michaelis-Menten kinetics kz[E]. [S] R= [S] + km a) What is the rate when [S] = km? b) What is the maximum rate (which is achieved when [S] >>> km) c) The rate of the reaction vs substrate concentration is given by the graph below. Indicate the VALUE of half of the maximum rate (Romex/2) and ku using the equations you derived in part a and...
Assuming that an enzyme catalyzed reaction follows Michaelis-Menten kinetics with a Km of 1 x 10-6 M. If the initial reaction rate (V0) is 0.1 μmol/min at 0.1 M, what would it be at 0.01 M, 10-3M, and 10-6 M?
Please answer all of those questions 7. Which of the followings is correct about the mechanisms of enzymatic reactions? General acid-base mechanism b. Metal elements mediated mechanism a. Covalent bonding mediated mechanism d. All above c. e. None a bove Which of the followings is correct about the meaning of the constant (Km) of Michaelis-Menten equation of enzyme catalyzed reactions? a. The constant and Michaelis-Menten equation is applicable only to the stage of reaction immediately after mixing of substrate(s) with...
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of Happyase for its substrate ICE is Km^s = 1mM. Happyase also acts on substrate CREAM and its Km^T =10mM. Is ICE or CREAM the preferred substrate for Happyase? Explain b. The rate constant k2 with substrate ICE is 2x10^4sec^ -1; with substrate CREAM, k2=4x10^5sec ^-1. Does Happyase use substrate ICE or substrate CREAM with greater catalytic efficiency? Show calculations and explain your answer
2) (5 marks) The enzyme Happyase follows simple Michaelis – Menten Kinetics a. The Km of Happyase for its substrate ICE is Kms = 1mM. Happyase also acts on substrate CREAM and its Km1 =10mM. Is ICE or CREAM the preferred substrate for Happyase? Explain b. The rate constant k2 with substrate ICE is 2x104sec-1; with substrate CREAM, k2=4x105sec-1. Does Happyase use substrate ICE or substrate CREAM with greater catalytic efficiency? Show calculations and explain your answer.
1.5 1.9 2.0 2.1 22 2.3 24 25 26 2.7 28 3) (a) An enzyme is used to convert a substrate at a temperature of 25°C. The Michaelis constant of this reaction is 0.042 mol dm3. The velocity of the reaction is 2.45 x 104 mol dm s when the substrate concentration is 0.890 mol dm3. Find the maximum velocity of this reaction? hint: vk2Er +161 and vmax (b) Plot v - vs -[S] for a standard enzymatic reaction that...
c. 0.6 sec d. 1.67 sec 16. Which of the following about Michaelis-Menten enzyme kinetics is CORRECT? a. It assumes that the maximum reaction rate is proportional to the catalytic constant multiplied by the total enzyme concentration. b. It assumes that the enzyme-substrate complex concentration remains steady state. c. KM is defined as the substrate concentration at which the velocity of the reaction is maximal, so the unit is M. d. The KM is assigned to each enzyme regardless of...