Question

1. Bacteroides thetaiotamicron is an important member of the gut microbiome. Recent experiments showed that gene expression in this organism within the mouse microbiome could be modulated precisely using

	a.	RT-PCR.
	b.	CRISPR-Cas9.
	c.	SDS-PAGE.
	d.	BLAST.

2. A reporter gene

	a.	allows for Southern and Northern blots to be probed.
	b.	can be assayed readily through its activity.
	c.	is a regulatory gene that activates other genes.
	d.	is a mutant gene that identifies the function through its defects.

Answer 1

1. Bacteroides thetaiotamicron is an important member of the gut microbiome. Recent experiments showed that gene expression in this organism within the mouse microbiome could be modulated precisely using

	a.	RT-PCR.
	b.	CRISPR-Cas9.
	c.	SDS-PAGE.
	d.	BLAST.

Answer:

PCR technique will be used for the outlining of expression of new gene from library and then this gene will be modulated in organism. So answer is a.

2. A reporter gene

	a.	allows for Southern and Northern blots to be probed.
	b.	can be assayed readily through its activity.
	c.	is a regulatory gene that activates other genes.
	d.	is a mutant gene that identifies the function through its defects.

Answer:

b. can be assayed readily through its activity.

Reporter genes have many functions as they can be used for activity of particular promotors and it can be used for quantitative analysis