Question

In order to determine the calcium content in milk powder, I did an experiment using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and Dye Binding Assay. However, the results obtained from both methods are different and inconsistent. Why is that so?

Answer 1

Before answering this Question let me make you comfortable with two terminoligies used within the question that is:

• Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and
• Dye Binding Assay

Basically Coupled Plasma Optical Emission Spectroscopy (ICP-OES) is  an analytical technique used for the detection of chemical elements.

whereas

Dye Binding Assay is quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.

Now, let us get back to your question.

Powder Milk is made by evaporating milk water content.

inductively coupled plasma optical emission spectrometry (VG-ICP-OES) was used for the determination of the iodine content in milk samples and he Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The dye forms a strong, noncovalent complex with the protein's carboxyl group by van der Waals force and amino group through electrostatic interactions.

Therefore Due to there characterstics of determinng different content in the mil the result which we discovered was different and incosistent.

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