Question

Your T.A. has streaked wild type and ADP-6 Acinetobacter on media containing succinate and para-hydroxybenzoate as sole carbon sources. As you can see, both strains grow on media containing succinate but only wild type grows on media containing para-hydroxybenzoate. Why is this control important, If this control doesn’t work, would it be worth performing the experiment?

Answer 1

control is important and worth it. As it is providing lots of information, wild type do grow on succinate containing media and parahydrobezoate containing media too. ADP acetobacter would not able to grow on para hydroxy benzoate which act as bactericidal its action is linked to membrane. this experiment shows that wild type have not greater lipid solubility to disturb the lipid bilayer of membrane. But ADP acetobacter have greater lipid solubility to disturb the lipid bilayer.  

If this control would not work, then we are not able to conclude that wild type and mutant type are different on the basis of their membrane.