you have cultured a throat swab specimen on a blood agar plate. you note the presence...
you have cultured a throat swab specimen on a blood agar plate. you note the presence of a clear halo around the colonies. these bacteria are exhibiting (a) endoenzyme production for hemolysis (b) exoenzyme production for hemolysis (c)exoenzyme production for for iron reduction (d) lactose fermentation (e) mannitol fermentation
Homework Answers
Blood agar plate contains sheep blood as one of it's constituents and if the bacteria grown on it secretes an exoenzyme that causes hemolysis a clear zone/Halo is formed around the colonies as the exoenzyme cleaves the RBCs in the blood. If it had been an endoenzyme it would have remained within the bacteria and will not be able to cleave the RBC and hence. No clear zone would have been observed.Lactose and mannitol fermenting bacteria can be differentially grown in McConkey and MSA respectively.Also if it had led to iron reduction then the area around would have turned black instead of clear as iron in reduced form is black. Therefore, answer is b) exoenzyme production for hemolysis.
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you have cultured a throat swab specimen on a blood agar plate.
you note the presence...
When we cultured the throat swab, why was the loop stabbed into the agar? to make...
When we cultured the throat swab, why was the loop stabbed into the agar? to make sure bacteria grow on the agar to protect catalases that are sensitive to the environment O to isolate colonies for hemolytic test O to surround the bacteria with mannitol salt O to protect hemolysins that are sensitive to oxygen
1. Human microbiota. What type of bacteria species are likely to be cultured from your throat...
1. Human microbiota. What type of bacteria species are likely to be cultured from your throat swabs on Blood agar? Why? 2. What is the purpose of the candle jar for the cultivation of the Blood Agar plate/throat swabs and what type of metabolism do you think you are targeting? 3.With reference to your two nasal swabs, what is the difference between the two culturing strategies: culture this swab on a mannitol-salt-agar plate and inoculate the nutrient broth containing 7.5%...
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab,...
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab, the purpose of stabbing the inoculum inot the agar (or placing in an anaerobic jar) is to: O provide reduced oxgyen tension for better growth obtain isolated colonies of respiratory flora clean off the inoculating loop enhance the beta-hemolytic activity of the pathogen - Question 11 Respiratory culture has the folloiwng results on BAP. 1. Alpha-hemolytic 2. G (+) lancet shaped cocci 3. catalase...
Microbiology - Gram Positive (ONLY answer) [Q1] A clear zone on blood agar is also known...
Microbiology - Gram Positive (ONLY answer) [Q1] A clear zone on blood agar is also known as _____________ hemolysis. [Q2] Which organism is the only one that can grow in the presence of 6.5% salt? [Q3] What color would you observe for an organism that is negative for mannitol fermentation?
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony...
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony type-II Colony type-l Colony type-III Gram stai Colony morphology, margin, elevation, color, etc. Hemolysis Table 3, Bacteria isolated from the rectal area on the EMB plate. Colony type-I Colony type-III Colony type-II Gram stain Colony morphology, margin, elevation, color, etc. Colony arrangement 4. Review questions d. In summary, what is the major purpose of this experiment? What are four reasons for knowing which microorganisms...
4 Photos 6. A throat swab was obtained from a paticnt with Streptococcal pharyngitis What type...
4 Photos 6. A throat swab was obtained from a paticnt with Streptococcal pharyngitis What type of expected en blood agar from the throat culture th can be either A or C 17. The species of Streptococous that causes pharymgits or "Strep throa" is IS. The pll indicator used in sugr feementation tubes is a. Neutral red b. Methyl red e. None of the above 19. Kligler's Iron agar, bocause of its composition, can be usod fo which of dhe...
can somrone help me write an introduction for each agar. thank you ? Introduction: Selective and...
can somrone help me write an introduction for each agar. thank you
?
Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
Q14. In the lab info it says that as a rule, most microbes can adjust to...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Can anyone help me by checking my answers? please indicate the answer in a numerical order....
Can anyone help me by checking my answers? please indicate the
answer in a numerical order. thank you.
Question 1 1 pts TRUE or FALSE: When we are working with our metabolic unknown samples, we should prepare a new TSA slant each day. True False Question 2 2 pts Which of the following is NOT a possible result of a test in which your unknown bacteria were introduced into a tube of phenol red lactose broth? Fermentation of lactose producing...
When we cultured the throat swab, why was the loop stabbed into the agar? to make sure bacteria grow on the agar to protect catalases that are sensitive to the environment O to isolate colonies for hemolytic test O to surround the bacteria with mannitol salt O to protect hemolysins that are sensitive to oxygen
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab, the purpose of stabbing the inoculum inot the agar (or placing in an anaerobic jar) is to: O provide reduced oxgyen tension for better growth obtain isolated colonies of respiratory flora clean off the inoculating loop enhance the beta-hemolytic activity of the pathogen - Question 11 Respiratory culture has the folloiwng results on BAP. 1. Alpha-hemolytic 2. G (+) lancet shaped cocci 3. catalase...
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony type-II Colony type-l Colony type-III Gram stai Colony morphology, margin, elevation, color, etc. Hemolysis Table 3, Bacteria isolated from the rectal area on the EMB plate. Colony type-I Colony type-III Colony type-II Gram stain Colony morphology, margin, elevation, color, etc. Colony arrangement 4. Review questions d. In summary, what is the major purpose of this experiment? What are four reasons for knowing which microorganisms...
4 Photos 6. A throat swab was obtained from a paticnt with Streptococcal pharyngitis What type of expected en blood agar from the throat culture th can be either A or C 17. The species of Streptococous that causes pharymgits or "Strep throa" is IS. The pll indicator used in sugr feementation tubes is a. Neutral red b. Methyl red e. None of the above 19. Kligler's Iron agar, bocause of its composition, can be usod fo which of dhe...
can somrone help me write an introduction for each agar. thank you
?
Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
Q14. In the lab info it says that as a rule, most microbes can adjust to a range of salt concentrations. 7/29/2019 Which cell structure allows microbes to physically withstand hypotonic environments? Cell wall MSA has two properties. It is both selective and differential. When bacteria grow on this media, there is a visible difference between bacteria that ferment mannitol, and the bacteria that do not See Figure 2 (a)-effects of selective/differential media. Q15. Which of the following is correct...
microbiology help
TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
Can anyone help me by checking my answers? please indicate the
answer in a numerical order. thank you.
Question 1 1 pts TRUE or FALSE: When we are working with our metabolic unknown samples, we should prepare a new TSA slant each day. True False Question 2 2 pts Which of the following is NOT a possible result of a test in which your unknown bacteria were introduced into a tube of phenol red lactose broth? Fermentation of lactose producing...
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