ZuoTi.Pro
Question

Suppose you have a cell line that expresses a protein whose structure you know but not...

Suppose you have a cell line that expresses a protein whose structure you know but not the function. Describe an experiment that would help you determine the protein’s function.

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

As you said in a question that you know the structure so I am thinking that you might know the amino acid sequence. So, you can try a BLAST tool to check homologous proteins and their fuction from reserch articles. To reconfirm your result you can knockdown the genes using siRNA or shRNA and check whether it affecting that similar pathway which are presnt in homologus proteins. You can also check fuction by inducing upregulating and downregulating proteins.

0 0
Add a comment
Know the answer?
Add Answer to:
Suppose you have a cell line that expresses a protein whose structure you know but not...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • You have isolated a lyophilized (in powder form) protein from a particular cell line. You have...

    You have isolated a lyophilized (in powder form) protein from a particular cell line. You have at least a gram of the material and a completely equipped laboratory including instruments, supplies, and reagents. List the steps you would take to determine the absorptivity of your protein in units of mL/mg·cm.

  • Suppose you are working on a gene that encodes a protein that triggers apoptosis of the...

    Suppose you are working on a gene that encodes a protein that triggers apoptosis of the cell (programmed cell death). In a certain cell, this gene is transcriptionally ON, that is, primary transcript is being made. Choose a mechanism by which to prevent or reduce the production of active apoptosis protein, and describe the specific properties of the new regulatory protein that the cell would have to make in order to do this.

  • 4. You have a protein that needs to bind ATP to be functional and has a...

    4. You have a protein that needs to bind ATP to be functional and has a KD for ATP of 10 μM. In you experiment your protein concentration is much less than that of ATP, and your final ATP concentration is 30 μM. A) What fraction of the protein is functional? Show your work. B) Suppose that you have a fluorescent ATP analog that can bind and activate your proteins that becomes quenched when it binds. Why would fluorescence change...

  • If a eukaryotic cell has synthesized a large quantity of a certain protein, you would know...

    If a eukaryotic cell has synthesized a large quantity of a certain protein, you would know that all of the following had occurred prior to protein formation, except: A. Repressor proteins were likely bound to all of the enhancer sequences and proximal elements of the gene for this protein. B. A transcription initiation complex was formed at the promoter region of the gene for this protein. C. Chromatin remodeling occurred in the region of the chromosome containing this gene for...

  • BINF4000 chapter 7, question 6 You have a protein sequence and you want to know iis...

    BINF4000 chapter 7, question 6 You have a protein sequence and you want to know iis structure in a short time. you perform BLAST and PSI-BLAST searches of the PDB and you find a sequence with 15% amino acid identity to your protein. The e-value is .5. Which of the following options would you choose to achieve your goal. A. Use X-ray crystallography B. Use NMR spectroscopy C. Submit your sequence to a protein structure prediction server that performs homology...

  • You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell...

    You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell lines require EGF in the culture media for growth). You suspect that EGF receptor signaling in this cell line is impaired, and you designate this a mutant cell line. As an assay for EGFR signaling, you have stably transfected this mutant cell line and a normal cell line with a luciferase-based reporter construct that is responsive to the transcription factor Elk-1. You want to...

  • SULR as the fow thermometer un 2. When a protein denatures in a cell, what causes...

    SULR as the fow thermometer un 2. When a protein denatures in a cell, what causes it to aggregate with other denatured cell proteins? (2pts) 3. How might a heat shock protein help a protein fold correctly in a cell under normal conditions? (2 pts). 4. The bacterial GroEL heat-shock protein and the plant Rubisco assembly proteins are said to be homologous proteins. What is it about these proteins that has caused them to be described as members of the...

  • Betergents h. What chemical would you add to refold this protein? 16. You wish to purify...

    Betergents h. What chemical would you add to refold this protein? 16. You wish to purify a novel protein from mammalian cells. You do not know this protein's size or charge, and are thus unsure of which steps to take. Fortunately, an antibody for this protein is available. You have two cell lines: one that expresses the protein, and one • that does not. . ) 4 5 III111 You run both cell lines on an SDS-PAGE (A) followed by...

  • 1-3 thank you Define and describe protein 1 degree, 2 degree, 3 degree, 4 degree structure...

    1-3 thank you Define and describe protein 1 degree, 2 degree, 3 degree, 4 degree structure c know amino acid sidechain know forces that determine protein 30 structure Define protein and domain families

  • Suppose you have a protein that can assume four possible conformations (A,B,C,D). When prepared, the protein...

    Suppose you have a protein that can assume four possible conformations (A,B,C,D). When prepared, the protein is equally likely to assume any of the conformations and will remain in that conformation. Suppose you prepare four protein samples and determine the conformation of each. 1) What is the probability of finding only one sample with the protein in structure A? 2) What is the probability of finding structure C in less than two samples (meaning either in zero or in one...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT