Question

I am having trouble with trying to figure out the genotypes of this.

Observe the photograph of a stained gel containing the pMCT118 amplified results from 6 unknown people. a. Lanes 1, 4, 7 and 10 contain and allelic ladder that corresponds to a sample mixture containing all 32 alleles at the PMCT118 locus 1 2 3 4 5 6 7 8 9 10 (alleles 13, 20, 30 and 44 are indicated). 44 b. Lanes 2, 3, 5, 6, 8 and 9 contain the result of the 6 unknown individuals. c. Fill in the table below with the genotype of the unknown individuals at this locus. (6 points) Individual Genotype d. or these 6 individuals, which are homozygous? Heterozygous? (4.5 pts) MARKER 5. Observe the photograph of a stained gel containing the pMCT118 STUDENT SAMPLES amplified results from pBR3227 4 unknown students with a different DNA Exu et al. I Hum Genet (2004) 49:169-171

Answer 1

The single band represents the homozygous genotype where as the two bands in a lane represents the heterozygous conditions.

A)

Look in the image for band:

44 43 7 8 9 10 || | | | || | | | | | | | | 2 3 4 5 6

The following genotype is found:

Individual	Genotype
2	29/44
3	31/16
5	24/36
6	30/42
8	18/27
9	30/43

D) heterozygoys individuals are: 2,3,5,6,8 and 9 .

None of the individuals are homozygous.