Question

Enzyme	Rf	Kda
BSA	0.16875	66.5
Lysozyme	0.65125	14.3
RNAse A	0.8225	13.7
Enzyme	Cm	Log U
BSA	1.35	0.16875
Lysozyme	5.21	0.65125
RNAse A	6.58	0.8225
total distance of gel	8

This is the data for the 12% gel acrylamide

Enzyme	Rf	Kda
BSA	0.22779043	66.5
Lysozyme	0.6833713	14.3
RNAse A	0.81548975	13.7
Enzyme	Cm	Log U
BSA	1	0.22779043
Lysozyme	3	0.6833713
RNAse A	3.58	0.81548975
total distance of gel	4.39

This is the data for the 15% gel acrylamide

Enzyme	Rf	Kda
BSA	0.39857143	66.5
Lysozyme	0.85714286	14.3
RNAse A	0.96428571	13.7
Enzyme	Cm	Log U
BSA	2.79	0.39857143
Lysozyme	6	0.85714286
RNAse A	6.75	0.96428571
total	7

This is the data for the 10% gel acrylamide

current ~25 mA/gel

The molecular weight of BSA 66.5kDA

The molecular weight of Lysozyme is 14.3 kDA

The molecular weight of RNase A 13.7 kDA

QUESTION: please help me construct a graph for RNAse A, BSA, and Lysozyme based on the % acrylamide as the (X-axis) and LOG U as the corresponding Y-axis. Please calculate the relative mobility based on how far the protein traveled on the gel. The values for the distance traveled is shown above.

Answer 1

Okay, so here goes the graph:

Logu Log U 10% 12% 15% % Acrylamide BSA - Lysozyme RNAse A

Now for the relative mobility (Rf), you have actually the Rf values, they are:

- 10%

BSA 0.39

Lysozyme 0.85

RNAse 0.96

- 12%

BSA 0.16

Lysozyme 0.65

RNAse 0.82

- 15%

BSA 0.22

Lysozyme 0.68

RNAse 0.81

Average Rf

BSA 0.25

Lysozyme 0.72

RNAse 0.86