Molarity = # of moles / volume of solution in litres
2 × 10-3 M = x / 1 × 10-3 L
X = 2 × 10-6 .
so , there will be 2 × 10-6 moles of ONP in given ONP stock and 5ml of PO4 buffer.
To collect data for an ONP standard curve, you need to prepare a series of test...
Table 2: Amounts of buffer, substrate, and enzyme added for lactase assay Tube 0.1 M 200 uM Lactase Total Abs ONP PO4 ONPG added (420 nm) Formed (mL) (mL) (mL) (mL) (umol) 4.00 0.00 6.0 5.50 0.00 0.50 6.0 .120 5.25 0.50 6.0 -127 5.00 0.50 6.0 4.50 0.50 6.0 0176 -186 3.50 0.50 6.0 221 1.50 0.50 6.0 396 1. Calculate moles of ONP in Table 1. Show your work for any tube other than Tube 1 and 2....
I’m trying to figure out how much of each i need to use to prepare
the stock buffer
CLIVILY DZ VEEN Tasks: prepare stock substrate in appropriate buffer o 10 mL 10 mM L-DOPA in 100 mM phosphate buffer, pH 6.0
Calculate the initial concentration of Fe3+ in test tubes 1-4. (you will need to take the dilution in to account) Calculate the initial concentration of SCN-in each of test tubes 1-4. (you will need to take the dilution in to account) 2. Label four 20 x 150 mm test tubes 1-4. Pour about 30 mL of 0.0020 M Fe(NO3)3 into a clean, dry 100-ml beaker. Pipet 5.0 mL of this solution into each of the four labeled test tubes. Use...
(1) How much amount (in milligrams) of riboflavin (MW=376.4 g/mol) you will need to prepare a 6.4x10-4M stock solution in 10 ml volume? (2) How much volume of the above stock solution will you need to prepare the riboflavin solutions of following concentrations: a. 0.059 mM in 25 ml b. 14 uM in 25 ml
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
help please! thank you!
2. In the Spectrophotometric analysis of your unknown compound you will create a standard curve with a stock solution of 0.400 M Cu. Determine the concentration of Cu in tubes 1 -4. Complete the values here and in this same table in your lab book. tube Contents Concentration of Cu ions (M Approximately 10 mL of1 M HNO 4.0 mL of 0.400 M Cu (stock standard) and 6.0 mL 1 M HNO 2 7.0 mL of...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
Results for QPCR report:
Absolute quantification (Standard curve) – Using a dilution
series of known template controls – Fluorescence is measured
against the standard curve – At least four points (in duplicate)
should be run for an accurate curve to be produced – The linearity
of the curve (Rsq value) should be close to 1
• To indicate the amplification efficiency is consistent across
all template concentrations
Your DNA standard 1 contains 1.0 x 106 copies of DNA, and you...
A standard curve was to be prepared from a stock of bromophenol blue. The stock concentration was 5 x 104 M. The standard concentrations required are listed in the table below, with the total volume of each to be 20 mL. 6. Table 1.2 Table for working out the volume of stock and water to be added for to make up the final concentrations required for Question 6 Vol. of stock concentration (mL) Vol. of water (mL) Final concentration (M)...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...