Question

5. A. (2 pts) You are growing NIH 3T3 (mouse embryonic fibroblasts) in a Petri dish. You provide these cells with a medium containing amino acids, glucose, vitamins, and salts necessary for their survival, but you run out of serum to supplement the medium. Without the addition of serum, what will MOST likely happen to these cells? A. The cells will die. B. The cells will continue to grow and proliferate as usual. C. The cells will remain viable, but will not grow and divide. D. The cells will proliferate at a higher than normal rate. E. None of the above. B. (2 pts) Please explain your answer in part A. 6.(2 pts) Mutations in the EGF receptor (EGF-R) that contribute to the development of cancer are MOST often due to A. Truncation of the ectodomain of the receptor. B. Elongation of the ectodomain of the receptor. C. Point mutations in the C-terminus of the receptor. D. Point mutations in the tyrosine kinase domain of the receptor. E. Cand D.

Answer 1

5. A. The cells will die .

B. Beacuse serum provide the cells with growth factors and hormones that will help the cells to grow and proliferate.

6. Truncation if ectodomain of the receptor. EGF-R has in frame deletions of exons 2-7 resulting in shorter Extracellular domain.