Question

Results Draw the pattern seen on the gel and label each line. Mark the anode and cathode. (+) block 0 0 0 hedral Kidney D 0 0 0 0 0 0 0 lungo 10 spleep ID mit I 1. Compare the LDH isoenzyme from different tissues and offer some possible explanations for the differences. 2. Compare the LDH isoenzyme pattern from different human serum samples. Offer some expla: nations for any differences noted. 3. Which tissue has the most LDH? kidney and Hvor neont The most different types of LDH?

Answer 1

Answer:

LDH is lactate dehydrogenase, a hydrogen transfer enzyme in cytoplasm of cells.Maximum fraction of ldh in human body is of LDH2.

Different types of LDH

a) LDH1 - H4

in myocardium and rbc

Elevated in myocardial infarction.

b) LDH2 - H3M1

Myocardium, rbc

c) LDH3 H2M2

Kidney, skeletal muscle

d) LDH4, H1M3

Kidney, skeletal muscle

e) LDH5, M4

Skeletal muscle, liver, ileum

Elevated in muscle disease and liver disease.

Liver isoenzyme is less sensitive than aminotransferase.

Half life of ldh is less than 6hrs.