answers:
a) the human p53 gene expresses not only 1 but 12
different p53 isoforms (proteins) which is due to the
alternative initiation of translation, alternate splicing,
alternative promotor usage. they are expressed in normal human
tissues expression but they are abnormally expressed in a wide
range of cancer type.
- p63 : expresses at least three
alternating spliced c-terminal isoforms
- p73 :expresses at least seven
alternating spliced c-terminal isoforms
they both share a significant structural and functional
similarity with the p53. +
- p53 ,
p53
,p53
40p53
40p53![\beta](//img.homeworklib.com/questions/778cb0a0-ebe8-11eb-8a0d-f7b0c2e2f68d.png?x-oss-process=image/resize,w_560)
40p53
133p53![\alpha](//img.homeworklib.com/questions/788b9a80-ebe8-11eb-942d-d53bbdca345b.png?x-oss-process=image/resize,w_560)
133p53
133p53![\gamma](//img.homeworklib.com/questions/77e4ff10-ebe8-11eb-a4ed-7f2582a1d846.png?x-oss-process=image/resize,w_560)
160p53
160p53
160p53![\gamma](//img.homeworklib.com/questions/77e4ff10-ebe8-11eb-a4ed-7f2582a1d846.png?x-oss-process=image/resize,w_560)
ans 2): mechanism by which each of
these are made:
133p53
,
133p53
and
133p53
:
transcription of p53 mRNA from the internal promoter
(intron 4) directed to alternative splicing of exon 9b.
160p53
,
160p53
and
160p53
: encoded by
133p53
,
133p53
and
133p53
mRNAs
by the alternative initiation of translation at ATG 160, which lies
within a sequence environment matching Kozack's consensus
criteria.
- p53
: alternative splicing of intron 9 (exon 9b)
- p53
:
truncation of p53 protein of the last 60 amino acids and containing
additional 15 amino acids.
40p53
,
40p53
and
40p53
: obtained by classic splicing of the C-terminus and
alternatively spliced c-terminal isoforms.
ans 3)
proteins which are translated from alternatively spliced mRNAs
will have difference in their amino acids sequences therefore they
will have difference in their functional properties.
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