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Partial Question 5 0.5/2 pts Match up the right answers. EDTA decreases pH, renatur SDS disrupts cell membrar 4 Sodium hydrox
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Answer #1

EDTA - Chelating Agent

Many enzymes require metal ion for their activity. Metal ion may either donate or accept electrons in an enzymatic reaction. It is important to carry out catalytic reaction. In nucleic acid extraction process, EDTA is used as chelating agent to chelate metal ions. So that RNAse and DNAse can be inhibited and prevent degradation of RNA and DNA.

SDS - Disrupts Cell Membrane

Sodium dodecyl sulfate (SDS) is an anionic detergent. It has a negative charge. They are surfactants because they decrease the surface tension of water. SDS totally disrupts membrane proteins and lipids by breaking interactions.

Sodium Hydroxide (NaOH) - Increases pH

Sodium Hydroxide is a strong base with pH 12. In water, it dissociates to produce Na+ and OH- ions. It decreases the hydrogen ion concentration and increase basicity. Thus increase the pH.

Sodium Acetate (CH3COONa) - Decreases pH

Sodium Acetate is a salt of weak acid. In water, Sodium Acetate dissociates in Ch3COO- and Na+ ions. Salt of weak acid and strong base added to water will result in acidic solution. Thus, it decreases pH.

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