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YPD 00 Strain Strain A Strain Strain B Strain strain sha/ NSS-27 blo 304-002
Observe the minus lysine plate for questions 4-6. Compare the strains to each other. 4) What do you observe? 5) What does t
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4. Strin C has not grown in -lysine plate. Where as other strains A, B have grown even in lysine minus plate.

5. Lysine is an essential aminoacid required for the formation of proteins in bacteria, it is also needed in the formation of peptidoglycan cell wall in bacteria. Strains A and B are able to synthesize lysine and hence they are able to survive and grow. Strain C is not able to synthesize lysin and hence it is not able to grow.

5. In the strains A and B, gene / genes involved in the synthesis of lysine are active and hence are able to synthesize lysine. In the strain C, one of genes required for the synthesis of lysine may have mutated and hence not able to synthesize lysine. Since lysine is very essential for bacterial survival, the strain C has not grown in lysine deficient medium.

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