Question

ect Question 7 0/1 pts The advantage of using topoisomerases to clone a PCR product is that topoisomerases can withstand the high temperatures of the PCR cycle. have proofreading activity. catalyze the formation of stronger phosphodiester bonds. can be covalently linked to the free ends of a TA vector, making for a very efficient process. All of the above are advantages None of the above are advantages

Answer 1

I believe the correct answer to be:

Option F) none of the above are advantages.

As topoisomerase are not used in cloning as their function is to wind and unwind the DNA which is generally not needed in the cloning process.

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