Question

.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar +Kan-Tet S Agar + Pen + Kan Agar +Kan + Amp Agar Tet + Amp SSSSSSS SSSSSSSS 2232 es + c • Which strain is the donor? Strain Which strain is the recipient? _Strain II What genetic information was donated (plasmid, chromosome or both) Plasmid Draw the genetic makeup of the recombinant Strain III in the oval at night: W . 3. Explain what antibiotic resistance means. Antibiotic resistance means that organism is not killed by a specific antibiotic due to gain of present of antibiotic resistant gene present either in genome or plasmid 4. If Strain II does not grow on a plate containing Tetracycline, is it resistant or susceptible to Tet? If strain II does not grow to tetracycline plate that it's become sensitive as it has loss its plasmid in which Tet resistant gene was present. rich Tetesi 3. Which strain (I or II) in this experiment contains an F plasmid? How do you know? plasmad? How do you know Strain I contains the F plasmid as F plasmid can donate from one organism to other organism and we seen plasmid from strain transformed to strain 6.If a bacterial strain is resistant to vancomycin, will it survive or be killed on a plate that contains enched agar and the addition of vancomycin?

Answer 1

Answer for 6th question

Bacterial strain resistant to vancomycin it added to a plate containing agar and vancomycin it will survive because it resistant to the vancomycin activities.