Question

Bacillus mepoterum Dichotomous Key for Gram + Bacteria Bm), Bacilus subtils (Bs), Enterococcus faecalis (Ef!, Staphylococcus aureus (sa), Staphylococcus soprophyticus (ss), Streptococcus rails (so). Streptococcus pneumoniae (Spl, Streptococcus pyogenes (Sov) Inoculate unknown on CNA: Did hemolysis occur?? No. Gamma or na growth Yes, hemolysis occurred El, Ss. Sa, Soy. So, Son Bm, Bs, Ef. 55 Inoculate chocolate agar- Perform catalase test-Positive? Was it gamma? Yes, BUBBLES! :D No, no growth No, no bubbles : Bm, B5 Sa, SS Ef. Ss Ef, Sa, Spn, Spy Is there white growth on the CNA blood plate? Is there white growth on the CNA blood plate? Inoculate chocolate agar- Perform catalase test-Positive? Yes No! Yes, Gamma! No No! Yes, Gamma Sa, SpnSpy Is there green growth on CNA plate? No! Reta! Yes! Alpha! Sa. Spn Positive MRVP Test? No!

Need help making my dichotomous key. The left side is okay, it's the right side I need help with. What test could I use to differentiate between Bm and Bs given this chart?

Answer 1

Dichotomous key :- Inoculate on CNA L. (Hemolysis ?) → No Gaminia, No Growth! BM,ⓇS [ Wasit Gamma No, No Growth Test [MR/ VP I positive Meo/vp8 BS I Negative Me / vpo BM Impor Both MRI VP Negative by the BND Important Important Into Only VP test positive shown by B