5. What is the role of isopropanol in the isolation of DNA and why do we...
6. What are the 3 types of RNAs discussed in this unit and what is the unique role that each of them has in protein synthesis? Type of RNA Role in Protein Synthesis MBNA ARNA ARNA 7. Why must a codon consist of three bases instead of two? (Hint: think about the number of amino acids that must be coded for) 8 Transcribe and translate the following template DNA sequence: 3-TAC GGGAATCGGTCTGITCAGATT-5' 9. Put the following steps of DNA Isolation...
What is the role of intronic DNA and why do you think there is so much of it? Is it truly “junk DNA” as it was originally named? What is the relationship between the size of the genome and complexity (ie: do more advanced organisms always have more genes?)
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
Please answer #3 using well #10 LIGATION/TRANSFORMATION/PLASMID ISOLATION RESULTS 1. What is a ligation? 2. What specifically does T4 DNA ligase do? 3. Was your ligation successful based on your electrophoresis results? Please include your electrophoresis image. Either tape below or staple to this sheet. How do you know? (2pts) 4. What is transformation? 5. Why was kanamycin added to the LB agar? 6. How do you know if your transformation of E.coli was successful? No Was your transformation of...
Human genomic DNA is around 3 billion bps. Why do we see a band around 10kb in 1% agarose gel electrophoresis? Did we do something wrong, damage the DNA and shorten it or something, what kb shall we expect to see it?
5. What do proteins do for your body? 6. What holds the sides of the DNA ladder together? 7. What are the sides of the DNA ladder made of? 8. What three parts make up a single nucleotide?
Why do we isolate for this disease and not for most other infectious diseases? What is especially concerning for active TB? . Do you feel that possible forced isolation should be done? If so, under what specific circumstances would this be imposed....all cases of active TB? I answered the question about TB test and she asks me tho above question after I answered the oringinal one. thx
Question 8 Why do you believe union membership in the US has declined? What role do you think that unions play in the workplace today?
Question Set:pDNA Isolation Data: Sample A: 0.02 Az00: 0.013 Dilution: To on 101 Concentration: 1. Lushal Questions: 1. What is RNase A and why was it added to the PDI Buffer? 2. Alcohol (EtOH) was added to the column Wash Buffer. Why? What does this do to the chromosomal DNA? 3. What structure collects the DNA by binding to the matrix? 4. Explain how detergents are used in the PD2 Buffer. 5. Name two methods for detecting DNA isolated in...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...