Question

Protein kinases are a class of enzymes that oftendunction in cancerous cells. Drugs can be used to Impede the activity of these enzymes by binding to them and blocking their catalysis. Shown below is data describing the binding behavior of several drugs to a kinase enzyme EGFR. Use these data to respond to the following questions.

Answer 1

Kd of a drug mostly calculated when half of the fraction is bound. So, in this case we have to search for concentration against Y = approximately 0.5 for each drug.

erlotinib - 500 nM

afatinib - 5 nM

gefitinib* - 100nM