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6. Isolating spontaneous yeast mutants is typically performed in haploid rather than diploid strains. What is...

6. Isolating spontaneous yeast mutants is typically performed in haploid rather than diploid strains. What is the main rationale for doing this?

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Isolation of spontaneous yeast mutants is generally performed in haploid strains. This is due to the fact that it is easier to identify recessive mutants in the haploid background. Haploid strains contain a single set of the genome. So, mutation of the single allele results in the phenotype for a recessive allele

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If we take a diploid yeast strain, both the alleles have to be mutated for the recessive phenotype to appear. So, the diploid M1 population is allowed to self to produce M2 so that recessive allele can attain homozygosity i.e. we have to look for the recessive phenotype in the next generation. If we use a haploid strain, we can observe the phenotype in the M1 generation itself.

Dominant mutations can appear in both homozygous and heterozygous conditions.

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