Homework Answers
The theoreticel PI would come out to be 8.20.
To calculate the pI, start with the lowest pH ex 1. calculate the net charge on the peptide on that pH. Then move the pH above the next pka but below the pka of the group next to it i.e in the middle. keep on doing this until you reach a net charge of 0. Then take the pka of both sides i.e higher and lower of that pH and divide it by 2 and you will get your approximate pI.
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6. Calculate the approximate pI of the peptide DSYRLKCF. Explain your work
Calculate the pI of a peptide containing Serine, Threonine, Arginine, Glutamic acid, Cysteine and Histidine? Peptide...
Calculate the pI of a peptide containing Serine, Threonine, Arginine, Glutamic acid, Cysteine and Histidine? Peptide written out is: STRETCH
What is the pI of the following peptide, Leu-Glu-His-Tyr? Show all your work, i.e. draw all...
What is the pI of the following peptide, Leu-Glu-His-Tyr? Show all your work, i.e. draw all the peptide structures (may be neatly hand drawn). ii) What is the overall charge at pH 3.5? Why? iii) What is the overall charge at pH 9? Why?
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
Calculate the pI for the free floating peptide that has the following sequence (show how you...
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Given is the peptide Ala-Lys-Lys-Asp-Phe 1) what is the pI for this peptide? show your calculations!...
Given is the peptide Ala-Lys-Lys-Asp-Phe 1) what is the pI for this peptide? show your calculations! 2) what is the net charge for this peptide at pH 6.5? show your calculations! Thank you :)
Estimate the pI for the peptide Arg-Met-His-Val-Glu. Please show work and explanation.
Estimate the pI for the peptide Arg-Met-His-Val-Glu. Please show work and explanation.
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
Using the following peptide, answer the following questions: HWESPGHAKCNKL. A.) This peptide has the following pKa...
Using the following peptide, answer the following questions: HWESPGHAKCNKL. A.) This peptide has the following pKa values associated with it: 2.1, 4.3, 6.0, 8.1, 9.3 & 10.7. What is the pI for this peptide? B.) What would be the overall charge on this peptide at: (i) pH 12? (ii) pH 7? C.) If this protein has the following modifications: one K side chain which is acetylated and the S side chain is phosphorylated. What would be the approximate pI for...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
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The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
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