Homework Answers
I believe the correct answers to be as follows:
1)Option C) No movement.
As the pI is the pH at which charge on peptide is zero and since the pH = pI in this case the zero charge would result in no movement by the aminoacid.
2) Option C) Towards the positive electrode.
As the pH increases from pI, the net charge becomes more and more negative and vice versa.
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
Add Answer to:
For each of the amino acids, predict the direction of migration during electrophoresis at the given...
The isoelectric point, pl, of the protein trypsin is 10.5, while that of clostripain is 4.8...
The isoelectric point, pl, of the protein trypsin is 10.5, while that of clostripain is 4.8 . What is the net charge of trypsin at pH 5.1 ? What is the net charge of clostripain at pH 2.5 ?! The isoelectric point of cysteine is 5.07 ; histidine , 7.59. During paper electrophoresis at pH 4.3 , toward which electrode does cysteine migrate? During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate?
Q.2. How to separate the mixture of the following amino acids (alanine pl = 6.01); lysine...
Q.2. How to separate the mixture of the following amino acids (alanine pl = 6.01); lysine (pl = 9.74) and aspartic acid (pl = 2.77) with the use of gel electrophoresis and the buffer of proper pH. Suggest buffer pH and present the order of investigated solute migrations. Buffer pH = Order of solute migrations toward cathode.
6. At neutral pH, which of the following amino acids has a net positive charge, which...
6. At neutral pH, which of the following amino acids has a net positive charge, which has a net negative charge, and which is neutral? (Hint: Draw the various charged forms of each amino acid before deciding.) a) Aspartic acid b) Histidine c) Valine 7. Which of the following forms of aspartic acid would you expect to predominate at low pH, neutral pH, and high pH? a) 10_char Line HOC-CH-CH-CO- +NH, b) ._ança do OC-CH-CH-CO- NH2 HOC-CH.CH +NH
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide...
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide move: Circle the correct answer. (2 marks) It will not move. Towards the positive electrode Towards the negative electrode Briefly Explain why: (2 marks) d) You are trying to separate 2 other peptides from your pentapeptide. Peptide 1 is made up of the amino acids: ARKS Peptide 2 is made up of the amino acids: ADES At what pH would you be able to...
1. What amino acids do the following abbreviations stand for? Draw the structure of each. a)...
1. What amino acids do the following abbreviations stand for? Draw the structure of each. a) lle b) Thr c) Gin 2. Name and draw the structures of the amino acids that fit these descriptions: a) Contains an isopropyl group b) Contains a secondary alcohol group 3. Which of the following objects is chiral? a) A pair of scissors b) A comb c) A drinking glass 4. What does the term achiral mean? Give two examples involving organic structures.. 5....
5. D-sorbital is found in 'sugar-free' chewing gum. It is formed by reducing D-glucose with NaBH Draw the structure of D-sorbital. s. (4 pts) Classify the following amino acid as polar, n...
5. D-sorbital is found in 'sugar-free' chewing gum. It is formed by reducing D-glucose with NaBH Draw the structure of D-sorbital. s. (4 pts) Classify the following amino acid as polar, non- polar, acidic, or basic: 6 (4 pts) The amino acid threonine (thr) has two chiral carbons. Draw a Fischer projection of the 2S,3S configuration of threonine. キ 7.(11 pts) Draw the tripeptide lys-asp-ser at physiological pH 8. (8 pts) The pK, values for aspartic acid are 2.10, 9.82...
Protein molecules in solution can be separated from each other by taking advantage of their net...
Protein molecules in solution can be separated from each other by taking advantage of their net charges. In the electric field between two electrodes, a positively charged particle moves toward the negative electrode and a negatively charged particle moves toward the positive electrode. This movement, known as electrophoresis, varies with the strength of the electric field, the charge of the particle, the size and shape of the particle, and the buffer/polymer gel combination through which the protein is moving. The...
TABLE 5.1 PK values for amino acids 2.36 Amino acid Alanine Arginine Asparagine Aspartic acid Cysteine...
TABLE 5.1 PK values for amino acids 2.36 Amino acid Alanine Arginine Asparagine Aspartic acid Cysteine Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine PK, PK₂ 2.34 9.69 2.17 9.04 2.02 8.80 1.88 9.60 1.96 10.28 2.19 9.67 2.17 9.13 2.34 9.60 1.82 9.17 9.60 9.60 2.18 8.95 2.28 1.83 9.13 1.99 10.60 2.219.15 2.09 9.10 2.83 9.39 2.20 9.11 2.32 9.62 2.36 9.21 5.56. Of the amino acids listed in Table...
#30,31,32,33,34 please ADDITIONAL PROBLEMS 18.3 AMINO ACIDS 18.30 The amino acids in most biological systems are...
#30,31,32,33,34 please
ADDITIONAL PROBLEMS 18.3 AMINO ACIDS 18.30 The amino acids in most biological systems are said to be a-L-acids. What does the prefix "a" mean? 18.31 What does the prefix "L" in a-L-acid mean? 18.38 18.32 What amino acids do the following abbreviations stand for? Draw the structure of each. (a) Ala (b) Cys (c) Asp 18.33 What amino acids do the following abbreviations stand for? Draw the structure of each. (a) Leu (b) Tyr (c) Asn 18.34 Name...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic R-groups, which have basic R-groups, and which have neutral R-groups? Write acidic", "basic" or "neutral next to each amino acid below. • Glycine: • Glutamic acid: • Arginine: • Tyrosine: 1. Circle all the charged carboxyl groups (carboxylate groups) in the amino acid structures below. | HN-CH-- HN-CH-C-ộ HN-CH-C ó Glycine (Gly) Glutamic acid or glutamate (Glu) C=NH2 NH2 Arginine (Arg) UUUUU Proteins Chemistry...
The isoelectric point, pl, of the protein trypsin is 10.5, while that of clostripain is 4.8 . What is the net charge of trypsin at pH 5.1 ? What is the net charge of clostripain at pH 2.5 ?! The isoelectric point of cysteine is 5.07 ; histidine , 7.59. During paper electrophoresis at pH 4.3 , toward which electrode does cysteine migrate? During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate?
Q.2. How to separate the mixture of the following amino acids (alanine pl = 6.01); lysine (pl = 9.74) and aspartic acid (pl = 2.77) with the use of gel electrophoresis and the buffer of proper pH. Suggest buffer pH and present the order of investigated solute migrations. Buffer pH = Order of solute migrations toward cathode.
6. At neutral pH, which of the following amino acids has a net positive charge, which has a net negative charge, and which is neutral? (Hint: Draw the various charged forms of each amino acid before deciding.) a) Aspartic acid b) Histidine c) Valine 7. Which of the following forms of aspartic acid would you expect to predominate at low pH, neutral pH, and high pH? a) 10_char Line HOC-CH-CH-CO- +NH, b) ._ança do OC-CH-CH-CO- NH2 HOC-CH.CH +NH
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide move: Circle the correct answer. (2 marks) It will not move. Towards the positive electrode Towards the negative electrode Briefly Explain why: (2 marks) d) You are trying to separate 2 other peptides from your pentapeptide. Peptide 1 is made up of the amino acids: ARKS Peptide 2 is made up of the amino acids: ADES At what pH would you be able to...
1. What amino acids do the following abbreviations stand for? Draw the structure of each. a) lle b) Thr c) Gin 2. Name and draw the structures of the amino acids that fit these descriptions: a) Contains an isopropyl group b) Contains a secondary alcohol group 3. Which of the following objects is chiral? a) A pair of scissors b) A comb c) A drinking glass 4. What does the term achiral mean? Give two examples involving organic structures.. 5....
5. D-sorbital is found in 'sugar-free' chewing gum. It is formed by reducing D-glucose with NaBH Draw the structure of D-sorbital. s. (4 pts) Classify the following amino acid as polar, non- polar, acidic, or basic: 6 (4 pts) The amino acid threonine (thr) has two chiral carbons. Draw a Fischer projection of the 2S,3S configuration of threonine. キ 7.(11 pts) Draw the tripeptide lys-asp-ser at physiological pH 8. (8 pts) The pK, values for aspartic acid are 2.10, 9.82...
TABLE 5.1 PK values for amino acids 2.36 Amino acid Alanine Arginine Asparagine Aspartic acid Cysteine Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine PK, PK₂ 2.34 9.69 2.17 9.04 2.02 8.80 1.88 9.60 1.96 10.28 2.19 9.67 2.17 9.13 2.34 9.60 1.82 9.17 9.60 9.60 2.18 8.95 2.28 1.83 9.13 1.99 10.60 2.219.15 2.09 9.10 2.83 9.39 2.20 9.11 2.32 9.62 2.36 9.21 5.56. Of the amino acids listed in Table...
#30,31,32,33,34 please
ADDITIONAL PROBLEMS 18.3 AMINO ACIDS 18.30 The amino acids in most biological systems are said to be a-L-acids. What does the prefix "a" mean? 18.31 What does the prefix "L" in a-L-acid mean? 18.38 18.32 What amino acids do the following abbreviations stand for? Draw the structure of each. (a) Ala (b) Cys (c) Asp 18.33 What amino acids do the following abbreviations stand for? Draw the structure of each. (a) Leu (b) Tyr (c) Asn 18.34 Name...
Name_Rachel A Date TITILLEELELE PROTEINSI Questions 1. Which amino acids used in this experiment have acidic R-groups, which have basic R-groups, and which have neutral R-groups? Write acidic", "basic" or "neutral next to each amino acid below. • Glycine: • Glutamic acid: • Arginine: • Tyrosine: 1. Circle all the charged carboxyl groups (carboxylate groups) in the amino acid structures below. | HN-CH-- HN-CH-C-ộ HN-CH-C ó Glycine (Gly) Glutamic acid or glutamate (Glu) C=NH2 NH2 Arginine (Arg) UUUUU Proteins Chemistry...
Most questions answered within 3 hours.
-
Industry standards suggest that 16% of new vehicles require
warranty service within the first year. Jones...
asked 1 minute ago -
1) Comment of this statement: “A compiler transforms high-level
language statements directly into object codes”.
asked 4 minutes ago -
Calculate the molality, mole-fraction and percent mass of 28.9M
HF at 25 degrees Celcius of the...
asked 13 minutes ago -
A developmental psychologist believes that children raised in
bilingual families will have higher verbal fluency at...
asked 19 minutes ago -
A fast food meal has 5660 kJ of energy. A person uses energy at
a rate...
asked 32 minutes ago -
The pKb for a generic amine(R-NH2)) in
aqueous solution is 6.30. What is its pKa?
asked 33 minutes ago -
The following reactions have the indicated equilibrium constants
at a particular temperature: N2(g) + O2(g) ⇌...
asked 35 minutes ago -
Please Help ASAP.
1Consider the below code which iterates over a linked
list of n nodes...
asked 51 minutes ago -
Determine the air to fuel ratio of:
Canadian natural gas, with 93.9% methane, 4.2% ethane, 0.3%...
asked 52 minutes ago -
A manufacturer of power tools claims that the average amount of
time required to assemble their...
asked 54 minutes ago -
"electron-withdrawing substituents on carbon encourage back
donation", then on the next page he says that "greater...
asked 1 hour ago -
On December 31, 2016, the shareholders’ equity section of the
balance sheet of R & L...
asked 1 hour ago