Question

Pyruvate kinase catalyzes the last reaction in the glycolytic pathway:

phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP

There are four different mammalian forms of pyruvate kinase. All catalyze the same reaction, but they differ in their response to the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).

The activity of two forms of pyruvate kinase was measured at various concentrations of PEP, as shown below. Activity was measured in the presence (blue circles) and absence (red circles) of F16BP.

Pyruvate Kinase Form 1 and Form 2 activity in the presence (blue circles) and absence (red circles) of F16BP.

Which of the following statements correctly describe the forms of pyruvate kinase?

Multiple answers:You can select more than one option

A

Form 1 is an allosteric enzyme.

B

Form 1 displays Michaelis-Menten (steady-state) kinetics.

C

Form 2 is an allosteric enzyme.

D

Form 2 displays Michaelis-Menten (steady-state) kinetics.

Answer 1

Option B and C are correct

B- Form 1 displays Michaelis-Menten (steady-state) kinetics.

C- Form 2 is an allosteric enzyme.

Michaelis menton enzyme show saturation hyperbola curve , enzyme form 1

While allosteric enzyme show cooperative binding and show sigmoidal curve. , enzyme form 2