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1st attempt Which of the following procedures is NOT used for polyclonal antibody generation? Choose one: A. Immunization of
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Answer #1

Answe: D (Fusion of B cells with immortalized tumor cells to form hybridoma)

Rationale: Generation of monoclonal antibody involve immunization of mice, followed by isolation of B cells from immunized mice. Isolated B cells allow to fused with immortalized tumor cells to generate hydridoma cells. Post fusion, hydridoma cells will be selected by HAT Medium (hypoxanthine-aminopterin-thymidine medium).

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