Answe: D (Fusion of B cells with immortalized tumor cells to form hybridoma)
Rationale: Generation of monoclonal antibody involve immunization of mice, followed by isolation of B cells from immunized mice. Isolated B cells allow to fused with immortalized tumor cells to generate hydridoma cells. Post fusion, hydridoma cells will be selected by HAT Medium (hypoxanthine-aminopterin-thymidine medium).
1st attempt Which of the following procedures is NOT used for polyclonal antibody generation? Choose one:...
1st attempt Which of the following is NOT correct when B cells produce antibodies? Choose one: A. The antigen binds to structured loops in the constant domains. B. Two classes of immunoglobulin light chain are produced. C. Each B cell only makes a single type of antibody. D. Five classes of immunoglobulin heavy chain are produced.
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
Please summarise the following content:(no word limit) EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
BIOL 1620o, Spring 2019 NAME (Last, Preferred First): Date: 45. What is the most important means by which capillay exchange occurs? 53. Which of the following organs does NOT contain lymphatic tissue? A. liver B. spleen C. lingual tonsils A. exocytosis B. diffusiorn C. ATP powered pumps D. facilitated diffusion E. pharyngeal tonsils. 46.According to Poiseuille's law A. blood flow is not related to resistance. B. ph of the blood influences flow C. viscosity of the blood is not related...