Question

Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Sample A contains 22.7 % thymine and sample B contains 29.3 % thymine. Assume the organisms contain normal double‑stranded DNA and predict the composition of the other bases.

Answer 1

& Both organism contull given Sample A Thymine contain = 22.7% Sample B Thymine contain = 29.3% organism contuin normal double stunded DNA. we have to calculate percentage of other bases lie. to feel contain of Adenine, Guanine & Cytosine In given organism's DNA. we know that DNA is composed of a bases A,T, G&C In Double strande DNA A binds with T with 2 hydrogen bonds & G binds with c with 3 hydrogen bonds. So that, ideally in double stranded DNA Content or percentage of base A=%T. & e=G For Sample A8 A = 22.7% that mean T= 22.7% ATT= 45.4%, so that Gre%= 100-45.6=54.5% [because (A+1% + (G+()%=100] G+c=54.5% that means G=54.5=27.25%,& Es 2 so that in Sample A:- Adenime=22.7% Guanine = 27.25% Thymine - 22-17., cytosine=27.25% For Sample B T = 29.3%. that means A=29.3% beknow that in double stranded DNA A+77# AITJGHE). TATT] + [G+6] = 100% so that G+2=100- ATT= 100-58-6=610G (A=29.3+T= 29.3=586] G+c= 41.4; so that G = 41.6=20.7%, & C= 20.7% I finally sample B contain:- denine-29.3%., Thymine=29.3% Guanine-2001%, Cytosine 20.7%