Albert is performing bacterial transformation. He has E. coli cells in 495 μl of CaCl2 transformation solution, and he adds 5 μl of 10 μg/ml plasmid. What is the final concentration of plasmid in his transformation reaction? Express your answer in μg/ml. Round your answer to the first decimal place.
Albert is performing bacterial transformation. He has E. coli cells in 495 μl of CaCl2 transformation...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
Need help filling in the chart and answering the questions that go along with it. I have added the procedure and the instructions as well as the "results" that are supposed to be used to fill in the chart. Thank you! We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...