Question

There are four proteolytic enzyme mechanisms that we studied in class. In a table similar to the one below, list every structural element that is involved in each of these four mechanism classes (up to 4 elements each; not each has as many as four). By each element, put the evidence that should be observed that shows its involvement and the catalysis type that each mediates (what tool from the mechanism toolbox is used?). A protease has been purified from a human retrovirus, which you call HRP1. You need to come up with a complete mechanism to help in developing a drug to combat this pathogen. Here is what you know:

Answer 1

enzyme class structural element Tool catalysis types serime traid 1)service 2)Histidine 3)Asp DIFP inactivation covalent cysteine 1)cysteine 2) Histidine 3) Glutomate or Asp Deprotonation of a thiol in enzyme actived site covalent low PH 1)PH acidic 2) environmental condition 3)Ap Acidic condition works for activation non - covalent acid - base mechanism Metalloenzyme 1)metal cordination 1) Histidine 2)cystenie 3)Aspartate coordination sphere formation dissociation of aud at biological PH values non - covalent