Homework Answers
1) Reference cuvet houses the reagent blank.
In reference cuvet distilled water can be used as reagent blank.
2) Mirror passes the beam to the detector.
From sample cuvet beam passes to detector through mirror.
3) sample cuvet holds the sample with a defined pathlength.
sample or compound under investigation is put in sample cuvet.
4) Amplifier increases the detector signal for ease of display and quantitation.
Amplifier amplifies the signal so that can be read easily and can be recorded on the recorder.
5)Beam chopper mirror that rotates to direct light either to the sample or to the reference cuvet.
This mirror chops the radiation and direct it to sample or reference cuvet.
6)Light source provides electromagnetic radiation to the ultravoilet, visible and infrared region of the spectrum .
The most suitable source of light for UV is the hydrogen or deuterium discharge lamp. For visible, tungsten filament is used as light source. Nernest glower is the most common source of IR. It consists of a rod of sintered mixture of the oxides of zirconium, ytterium, and erbium.
7)Detector typically a photomultiplier tube that generates an electric current proportional to photon flux.
Detector detects sperctral signal and convert it into electrical signal.
8) monochromator is wavelength selector
Monochromator is a device for getting radiation of single frequency.
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