Question

QUESTION 4 The adenylate pool (ATP+ADP+AMP) for two cell types is shown below. Calculate the energy charge of each and determine which is the more anabolic cell type. liver cells cancer cells ATP 3.4 mm 1.25 mm ADP 1.3 mm 0.35 mM AMP 0.3 mm 0.12 mm energy charge metabolic state 0.81 0.83 0.85 0.87 0.89 0.91 Less anabolic More anabolic QUESTION 5 Adenylate kinase is a ubiquitous en through B-phosphoryl transfer from aintain short term ATP pools during high energy demand DP to synthesize ATP at the cost of producing one AMP. Indor normal adenine nucleotide hall batalyze the reverse reaction in order to recycle AMD for

Answer 1

Energy charge (ATP] + ₂ [ADP ATP + ADP TAMP Ec of liver = ll [3.4] + 3.4 + 1.3 + 3.4 + 0.65 [1-3 0-3 5 4.03 -0.81 E-c of cancer = Concer Cell 1-25+ { [0:35] 7.25 +0.35+ 0.12 1.25 to 175 +-72 7.425 - - 0.828 1.72

Hence energy charge of cancer cell is higher. And it proved Cancer cell is more anabolic because high energy charge inhibit ATP generating (Catabolic) and induced ATP utilizing (Anabolic) pathway.