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3. Give two ways reverse phase chromatography differs from normal chromatography: Lab 236 Column Chromatography Name: Section
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Answer #1

3.

Normal phase Reverse phase

Mobile phase: Non-Polar

Stationary phase: Polar

Mobile phase: Polar

Stationary phase: Non-Polar
Normal phase is based on an adsorptive mechanism, and is used for the analysis of hydrophobic solutes that are readily soluble in organic solvents. Reverse phase is useful for separating compounds that have hydrophobic moieties and also that do not have a pre-dominant polar character.

4. Advantages of column chromatography over paper chromatography

All kinds of complex mixtures can be separated

Analytes can be separated and as well as reused

Broad range of mobile phase can be used

Any amount of mixture can be conveniently separated

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