Question

Sonicators can be used to release protein from bacteria without worrying about detergents interfering with the protein folding. True False Bacteria can be used to make eukaryotic protein. True False Wells in polymerized gels are created using combs. True False pH changes will affect the visibility of p-nitrophenol. True False Phosphate buffered saline will cause proteins to unfold. True False

Answer 1

Answer :

Question number 10 : The given statement is True. As sonication/sonoporation uses the ultrasonic waves for the disruption of cell membranes which releases the protein molecules.

Question number 11 : The given statement is TRUE. Production of eukaryotic protein in bacteria can be acheived by inserting a cDNA fragment in to bacteria through expression vectors.

Question number 12 : The given statments is TRUE. After pouring the componts of stacking gel, A comb is inserted into the casket which upon polymerisation leaves the shape of wells that can be used to load samples.