Question

Polymerase chain reaction (PCR) can be used for many different purposes, including determining paternity. PCR amplifies specific DNA sequences from complex mixtures and can be used to amplify sequences that although they may not have any known function may have several unique sizes and these different forms are inherited according to the Law of Segregation. Below is a diagram of an agarose gel of PCR samples from a mother, and several children. Which letters represent children that could be blologically related to the mother? Mother A B C D A, B, and To o o C B, C, and D All of the children could be related to the mother

Answer 1

In the agarose gel plate given above,the relatedness is analysed by matching of fragment length between mother and her children's genome.

In case there is no matching with the mother,so A is not the child.

In case B the lower fragment matches with the mother,so he is the child. The other unmatched fragment may be of his father.

In case of C the fragment matches with that of the mother,so he is also the child.

In case D like that of B, lower fragment matches. So he is the child.

Therefore the option 2.BCD will be the right option.

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