Please answer all part of the question
(3.2) I have a sam file called “sequences.sam” and a genomic feature file called “features.bed”. I would like to annotate all the sequences in the sam file with the bed file (overlapping features). Can you write down the commands needed using samtools and bedtools?
(3.3) Can you obtain at least two mRNA isoforms of TP53 from NCBI or other online resources? Generate a bed file containing both, color them differently and visualize them on UCSC genome browser? Take a screenshot of the visualization
3.2The command –bedtools OVERLAP
bedtools intersect -asam sequences.sam –b features.bed
3.3 Δ133p53β and Δ133p53γ two mRNA isoforms of TP53 from NCBI
Please answer all part of the question (3.2) I have a sam file called “sequences.sam” and...
Can you obtain at least two mRNA isoforms of TP53 from NCBI or other online resources? Generate a bed file containing both, color them differently and visualize them on UCSC genome browser? Take a screenshot of the visualization.
Part I— Just Bad Luck? Brrrring! Brrrring! Jane checked the caller ID on her phone. “Sam! Great!” she thought. It was always nice to get a call from her older brother. But a little twinge of worry tugged at her. It was just a couple of weeks ago that he had mentioned making an appointment with his doctor about some abdominal pain he had been having. “Hi Sam! It’s great to hear from you,” Jane answered. “Hi Jane. Well I...