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7) When Crick et al. plated large numbers of T4, with suppression mutations only, on K2...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of TI-phage sensitive E. coli to plates containing high concentrations of TI phase and found a small number of phage-resistant colonies showed that: A) a selecting agent can affect mutation rate in E. coli. B) mutations can arise spontaneously before exposure to the selecting agent. C) mutations in E. coli occur at a relatively high frequency, D) the mutation rate in E. coli fluctuates greatly...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of TI-phage sensitive E. coli to plates containing high concentrations of TI phase and found a small number of phage-resistant colonies showed that: A) a selecting agent can affect mutation rate in E. coli. B) mutations can arise spontaneously before exposure to the selecting agent. C) mutations in E. coli occur at a relatively high frequency, D) the mutation rate in E. coli fluctuates greatly...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
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