Question

7.) (5 points) ?nRH is a hypothalamic neuropeptide that controls mammalian reproduction. Total RNA was extracted from the rat hypothalamus tissue and Northern blot analysis was performed with liga probes complementary to the 5 'end of ?nRH mRNA to detect this mRNA. Lane 1 shows blot of control in vitro synthesized ?nRH mRNA (560, Lane 2 shows blot of the total RNA extract. 2 4300 nt Explain why lane 2 displays a large smear with several major bands showing above the mRNA band. What is the mechanism that generates these additional bands? O RNA

Answer 1

Thai happens because the GnRh mRNA gets alternatively splices - 1.2 kb and 2.16 kb. They do not vary in their 5' region. Hence, these transcript can bind to the oligo probes.l